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KMID : 0545120150250020302
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 2 p.302 ~ p.306
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VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy
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Lee Mi-Ae
Kim Jeong-A
Shin Mee-Young
Lee Jeong-K.
Park Soon-Jung
Lee Kyu-Ho
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Abstract
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VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.
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KEYWORD
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Vibrio vulnificus, Metalloprotease VvpM, Necroptosis, Autophagy
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